Subject:
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NCEBP 2: Evaluation of complex medical interventions N4i 4: Auto-immunity, transplantation and immunotherapy NCMLS 1: Infection and autoimmunity NCMLS 1: Infection and autoimmunity N4i 4: Auto-immunity, transplantation and immunotherapy NCMLS 2: Immune Regulation NCMLS 4: Energy and redox metabolism IGMD 8: Mitochondrial medicine |
Organization:
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Cell Biology (UMC) Rheumatology Biochemistry (UMC) Dermatology |
Journal title:
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Biochimica et Biophysica Acta. Molecular Cell Research
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Abstract:
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DMPK, the product of the mutated gene in myotonic dystrophy type 1, belongs to the subfamily of Rho-associated serine-threonine protein kinases, whose members play a role in actin-based cell morphodynamics. Not much is known about the physiological role of differentially localized individual DMPK splice isoforms. We report here that prominent stellar-shaped stress fibers are formed during early and late steps of differentiation in DMPK-deficient myoblast-myotubes upon complementation with the short cytosolic DMPK E isoform. Expression of DMPK E led to an increased phosphorylation status of MLC2. We found no such effects with vectors that encode a mutant DMPK E which was rendered enzymatically inactive or any of the long C-terminally anchored DMPK isoforms. Presence of stellar structures appears associated with changes in cell shape and motility and a delay in myogenesis. Our data strongly suggest that cytosolic DMPK participates in remodeling of the actomyosin cytoskeleton in developing skeletal muscle cells. This article is part of a Special Issue entitled: 11th European Symposium on Calcium.
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