Reduced atherosclerosis and inflammatory cytokines in apolipoprotein-E-deficient mice lacking bone marrow-derived interleukin-1alpha.
Publication year
2011Source
Biochemical and Biophysical Research Communications, 405, 2, (2011), pp. 197-203ISSN
Publication type
Article / Letter to editor
Display more detailsDisplay less details
Organization
Internal Medicine
Journal title
Biochemical and Biophysical Research Communications
Volume
vol. 405
Issue
iss. 2
Page start
p. 197
Page end
p. 203
Subject
N4i 1: Pathogenesis and modulation of inflammationAbstract
OBJECTIVE: Interleukin (IL)-1alpha and IL-1beta are products of macrophages, endothelial cells and vascular smooth muscle cells; moreover, each of these cell types is affected by the pro-inflammatory properties of both IL-1's. Whereas several studies demonstrate the proatherogenic properties of IL-1beta, the role of IL-1alpha in atherogenesis remains unclear. We assessed whether IL-1alpha and IL-1beta from tissue resident vascular cells or emigrating bone marrow-derived cells promote the development of atherosclerosis in apoE-/- mice and determined the effect of selective macrophage IL-1alpha or IL-1beta deficiency on degradation of LDL and cytokine production. METHODS: We generated strains of double knock-out (KO) mice (apoE-/-/IL-1alpha-/- and apoE-/-/IL-1beta-/-) and created chimeras consisting of apoE-/- mice reconstituted with bone marrow-derived cells from apoE-/-/IL-1+/+, apoE-/-/IL-1alpha-/- and apoE-/-/IL-1beta-/-. RESULTS: The areas of aortic sinus lesions were lower in either double KO mice compared to solely apoE-/- mice, despite higher non-HDL cholesterol levels. Importantly, selective deficiency of IL-1alpha or IL-1beta in bone marrow-derived cells inhibited atherogenesis to the same extent as in double KO mice without affecting plasma lipids. Aortic sinus lesions in apoE-/- mice transplanted with IL-1beta-/- or IL-1alpha-/- cells were 32% and 52% lower, respectively, than in IL-1+/+ transplanted mice. Ex vivo, isolated IL-1alpha-/- macrophages from atherosclerotic mice degraded LDL and secreted IL-6, TNFalpha and IL-12 similarly to IL-1+/+ macrophages; however, IL-1alpha deficient macrophages secreted reduced levels of IL-1beta (-50%) and 2-3-fold higher levels of the anti-inflammatory cytokine IL-10. CONCLUSION: We show for the first time that it is IL-1alpha from bone marrow-derived cells that accelerates atherogenesis in apoE-deficient mice rather than constitutive IL-1alpha in vascular cells, possibly by increasing the inflammatory cytokine profile of macrophages.
This item appears in the following Collection(s)
- Academic publications [245186]
- Faculty of Medical Sciences [93207]
Upload full text
Use your RU credentials (u/z-number and password) to log in with SURFconext to upload a file for processing by the repository team.