Novel conditionally immortalized human proximal tubule cell line expressing functional influx and efflux transporters.
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Publication year
2010Source
Cell and Tissue Research, 339, 2, (2010), pp. 449-57ISSN
Annotation
01 februari 2010
Publication type
Article / Letter to editor
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Organization
Paediatrics - OUD tm 2017
Pharmacology-Toxicology
Laboratory of Genetic, Endocrine and Metabolic Diseases
IMM - Institute for Molecules and Materials
Journal title
Cell and Tissue Research
Volume
vol. 339
Issue
iss. 2
Page start
p. 449
Page end
p. 57
Subject
IGMD 9: Renal disorder; NCMLS 4: Energy and redox metabolism; NCMLS 5: Membrane transport and intracellular motility; Synthetic Organic Chemistry; Laboratory Medicine Radboud University Medical CenterAbstract
Reabsorption of filtered solutes from the glomerular filtrate and excretion of waste products and xenobiotics are the main functions of the renal proximal tubular (PT) epithelium. A human PT cell line expressing a range of functional transporters would help to augment current knowledge in renal physiology and pharmacology. We have established and characterized a conditionally immortalized PT epithelial cell line (ciPTEC) obtained by transfecting and subcloning cells exfoliated in the urine of a healthy volunteer. The PT origin of this line has been confirmed morphologically and by the expression of aminopeptidase N, zona occludens 1, aquaporin 1, dipeptidyl peptidase IV and multidrug resistance protein 4 together with alkaline phosphatase activity. ciPTEC assembles in a tight monolayer with limited diffusion of inulin-fluorescein-isothiocyanate. Concentration and time-dependent reabsorption of albumin via endocytosis has been demonstrated, together with sodium-dependent phosphate uptake. The expression and activity of apical efflux transporter p-glycoprotein and of baso-lateral influx transporter organic cation transporter 2 have been shown in ciPTEC. This established human ciPTEC expressing multiple endogenous organic ion transporters mimicking renal reabsorption and excretion represents a powerful tool for future in vitro transport studies in pharmacology and physiology.
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