Attenuation of invariant natural killer T-cell anergy induction through intradermal delivery of alpha-galactosylceramide.
Publication year
2010Source
Clinical Immunology, 136, 3, (2010), pp. 364-374ISSN
Annotation
1 september 2010
Publication type
Article / Letter to editor

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Organization
Laboratory of Hematology
Journal title
Clinical Immunology
Volume
vol. 136
Issue
iss. 3
Page start
p. 364
Page end
p. 374
Subject
NCMLS 2: Immune Regulation; ONCOL 3: Translational researchAbstract
CD1d restricted, alpha-galactosylceramide (alphaGC) responsive invariant (i)NKT cells positively regulate immune responses. Both intravenous and intradermal administered alphaGC are known to activate iNKT cells. iNKT cells become unresponsive to a second intravenous alphaGC injection, whereas no data are available regarding potential anergy upon intradermal administration. Here, comparative analysis of two intradermal versus two intravenous injections in mice demonstrated that iNKT cell anergy was prevented by intradermal injection and when combined with a vaccine, superior tumor protection afforded by intradermally administered alphaGC. Moreover, human skin dendritic cells (DC) took up intradermally injected alphaGC and activated iNKT cells upon migration, while iNKT cells in human skin-draining lymph nodes expanded in response to alphaGC presented either by exogenously added DC or by CD1d positive antigen presenting cells in the lymph nodes. In conclusion, glycolipids such as alphaGC may greatly improve the efficacy of skin immunization strategies, targeting cutaneous and lymph node DC.
This item appears in the following Collection(s)
- Academic publications [204951]
- Faculty of Medical Sciences [81049]
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