Identification of a major rif transcript common to gametocytes and sporozoites of Plasmodium falciparum.
Publication year
2010Source
Malaria Journal, 9, (2010), pp. 147ISSN
Publication type
Article / Letter to editor
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Organization
Medical Microbiology
Journal title
Malaria Journal
Volume
vol. 9
Page start
p. 147
Page end
p. 147
Subject
N4i 3: Poverty-related infectious diseases; NCMLS 1: Infection and autoimmunityAbstract
BACKGROUND: The Plasmodium falciparum parasite is transmitted in its sexual gametocyte stage from man to mosquito and as asexual sporozoites from mosquito to man. Developing gametocytes sequester preferentially in the bone marrow, but mature stage gametocytes are released to the bloodstream. Sexual stage parasite surface proteins are of interest as candidate target antigens for transmission blocking vaccines. METHODS: In this study, the transcript profiles of rif and var genes, known to encode surface antigens in asexual blood stage parasites, were investigated at different stages of 3D7/NF54 gametocytogenesis and in sporozoites. RESULTS: Gametocytes exhibited a rif transcript profile unlinked to the rif and var transcript profile of the asexual progenitors. At stage V, mature gametocytes produced high levels of a single rif gene, PF13_0006, which also dominated the rif transcript profile of sporozoites. All var genes appeared to be silenced in sporozoites. CONCLUSIONS: The most prominent variant surface antigen transcribed in both gametocytes and sporozoites of 3D7/NF54 is a single variant of the RIFIN protein family. This discovery may lead to the identification of the parasites binding ligands responsible for the adhesion during sexual stages and potentially to novel vaccine candidates.
This item appears in the following Collection(s)
- Academic publications [238586]
- Electronic publications [122848]
- Faculty of Medical Sciences [90409]
- Open Access publications [97830]
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