Keratinocytes drive melanoma invasion in a reconstructed skin model.
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Publication year
2010Source
Melanoma Research, 20, 5, (2010), pp. 372-80ISSN
Annotation
01 oktober 2010
Publication type
Article / Letter to editor
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Organization
Dermatology
Pathology
Journal title
Melanoma Research
Volume
vol. 20
Issue
iss. 5
Page start
p. 372
Page end
p. 80
Subject
NCMLS 1: Infection and autoimmunity; ONCOL 3: Translational researchAbstract
Melanoma progression is a multistep progression from a common melanocytic nevus through the radial growth phase, the invasive vertical growth phase finally leading to metastatic spread into distant organs. Migration and invasion of tumor cells requires secretion of proteases to facilitate remodeling of the extracellular matrix including basement membranes. Here we used a reconstructed skin model to investigate melanoma growth and invasion in vitro. Using this model we show that the dermoepidermal basement membrane prevents the invasion of metastatic melanoma BLM and MV3 cells in the absence of a stratified epidermis. In the reconstructed skin model, matrix metalloproteinase-9, a protease activated early in melanoma development, is secreted by the keratinocytes and subsequently activated by an unknown soluble factor secreted by the melanoma cells. The dynamic interplay between keratinocytes and melanoma cells is further shown by an altered growth pattern of melanoma cells and the finding that a reconstructed epidermis induces invasion. Overall, our findings show that the invasive behavior of melanoma cells is determined by the melanoma cells themselves, but that the interplay between surrounding keratinocytes and the melanoma cells plays an important role in melanoma invasion.
This item appears in the following Collection(s)
- Academic publications [245400]
- Electronic publications [132942]
- Faculty of Medical Sciences [93207]
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