The influence of nanoscale grooved substrates on osteoblast behavior and extracellular matrix deposition.
Fulltext:
87800.pdf
Embargo:
until further notice
Size:
1.932Mb
Format:
PDF
Description:
Publisher’s version
Publication year
2010Source
Biomaterials, 31, 12, (2010), pp. 3307-16ISSN
Annotation
01 april 2010
Publication type
Article / Letter to editor
Display more detailsDisplay less details
Organization
Dentistry
Tumorimmunology
Journal title
Biomaterials
Volume
vol. 31
Issue
iss. 12
Page start
p. 3307
Page end
p. 16
Subject
NCMLS 2: Immune Regulation; NCMLS 3: Tissue engineering and pathologyAbstract
To fight bone diseases characterized by poor bone quality like osteoporosis and osteoarthritis, as well as in reconstructive surgery, there is a need for a new generation of implantable biomaterials. It is envisioned that implant surfaces can be improved by mimicking the natural extracellular matrix of bone tissue, which is highly a organized nano-composite. In this study we aimed to get a better understanding of osteoblast response to nanometric grooved substrates varying in height, width and spacing. A throughput screening biochip was created using electron beam lithography. Subsequently, uniform large-scale nanogrooved substrates were created using laser interference lithography and reactive ion etching. Results showed that osteoblasts were responsive to nanopatterns down to 75 nm in width and 33nm in depth. SEM and TEM studies showed that an osteoblast-driven calcium phosphate (CaP) mineralization was observed to follow the surface pattern dimensions. Strikingly, aligned mineralization was found on even smaller nanopatterns of 50 nm in width and 17 nm in depth. A single cell based approach for real time PCR demonstrated that osteoblast-specific gene expression was increased on nanopatterns relative to a smooth control. The results indicate that nanogrooves can be a very promising tool to direct the bone response at the interface between an implant and the bone tissue.
This item appears in the following Collection(s)
- Academic publications [238441]
- Electronic publications [122537]
- Faculty of Medical Sciences [90373]
Upload full text
Use your RU credentials (u/z-number and password) to log in with SURFconext to upload a file for processing by the repository team.