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Publication year
2010Source
Journal of Leukocyte Biology, 87, 2, (2010), pp. 283-9ISSN
Annotation
01 februari 2010
Publication type
Article / Letter to editor
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Organization
Tumorimmunology
Journal title
Journal of Leukocyte Biology
Volume
vol. 87
Issue
iss. 2
Page start
p. 283
Page end
p. 9
Subject
NCMLS 3: Tissue engineering and pathologyAbstract
Previously, we demonstrated that several TLRs are expressed on cord blood-derived USSC. Stimulation of USSC with TLR agonists resulted in a marked increase of IL-6 and IL-8 production. Interestingly, TNF was undetectable after TLR stimulation, which appeared to be a result of an inactivated TNF promoter in USSC. Here, we elaborate this study by demonstrating that although USSC do not produce TNF, they are susceptible to TNF stimulation, resulting in NF-kappaB translocation and cytokine production. Additionally, we compared different stem cell sources for their ability to produce TNF. Interestingly, we found that the TNF promoter in BM-MSC is inactivated as well. Like USSC, they are able to respond to TNF stimulation, but they are not able to produce TNF, even not after LPS stimulation. This limited cytokine response in combination with the well-studied immunosuppressive properties of MSC makes these cells ideal for immune-suppressive treatment modalities such as graft-versus-host disease.
This item appears in the following Collection(s)
- Academic publications [238441]
- Electronic publications [122519]
- Faculty of Medical Sciences [90373]
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