Vitamin A equivalency and apparent absorption of beta-carotene in ileostomy subjects using a dual-isotope dilution technique.

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Publication year
2010Source
British Journal of Nutrition, 103, 12, (2010), pp. 1836-1843ISSN
Annotation
1 juni 2010
Publication type
Article / Letter to editor

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Organization
Gastroenterology
IMM - Institute for Molecules and Materials
Pharmacology-Toxicology
Journal title
British Journal of Nutrition
Volume
vol. 103
Issue
iss. 12
Page start
p. 1836
Page end
p. 1843
Subject
IGMD 2: Molecular gastro-enterology and hepatology; NCMLS 5: Membrane transport and intracellular motility; Synthetic Organic ChemistryAbstract
The objective was to quantify the vitamin A equivalency of beta-carotene in two diets using a dual-isotope dilution technique and the apparent beta-carotene absorption as measured by the oral-faecal balance technique. Seventeen healthy adults with an ileostomy completed the 4-week diet-controlled, cross-over intervention study. Each subject followed both diets for 2 weeks: a diet containing vegetables low in beta-carotene content with supplemental beta-carotene in salad dressing oil ('oil diet'; mean beta-carotene intake 3.1 mg/d) and a diet containing vegetables and fruits high in beta-carotene content ('mixed diet'; mean beta-carotene intake 7.6 mg/d). Daily each subject consumed a mean of 190 microg [13C10]beta-carotene and 195 microg [13C10]retinyl palmitate in oil capsules. The vitamin A equivalency of beta-carotene was calculated as the dose-corrected ratio of [13C5]retinol to [13C10]retinol in serum. Apparent absorption of beta-carotene was determined with oral-faecal balance. Isotopic data quantified a vitamin A equivalency of [13C10]beta-carotene in oil of 3.6:1 (95 % CI 2.8, 4.6) regardless of dietary matrices differences. The apparent absorption of (labelled and dietary) beta-carotene from the 'oil diet' (30 %) was 1.9-fold higher than from the 'mixed diet' (16 %). This extrinsic labelling technique can measure precisely the vitamin A equivalency of beta-carotene in oil capsules, but it does not represent the effect of different dietary matrices.
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- Faculty of Medical Sciences [81054]
- Faculty of Science [32353]
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