The C-type lectin DC-SIGN internalizes soluble antigens and HIV-1 virions via a clathrin-dependent mechanism.

Fulltext:
81388.pdf
Embargo:
until further notice
Size:
328.5Kb
Format:
PDF
Description:
publisher's version
Publication year
2009Source
European Journal of Immunology, 39, 7, (2009), pp. 1923-1928ISSN
Publication type
Article / Letter to editor

Display more detailsDisplay less details
Organization
Tumorimmunology
Cell Biology (UMC)
Journal title
European Journal of Immunology
Volume
vol. 39
Issue
iss. 7
Page start
p. 1923
Page end
p. 1928
Subject
NCMLS 2: Immune Regulation; NCMLS 4: Energy and redox metabolism; NCMLS 5: Membrane transport and intracellular motility; ONCOL 3: Translational researchAbstract
Dendritic cells (DC), professional Ag-presenting cells located in mucosae and lymphoid organs, operate at the interface of innate and adaptive immunity and are likely the first cells to encounter invading HIV-1. Although the C-type lectin DC-Specific ICAM-3-grabbing non-integrin (DC-SIGN) binds to several viruses, including HIV-1, its direct involvement in viral entry remains controversial. Despite its central role in DC function, little is known about the underlying molecular mechanism(s) of DC-SIGN-mediated Ag uptake. Here, we analyzed the early stages of DC-SIGN-mediated endocytosis and demonstrate that both membrane cholesterol and dynamin are required. Confocal microscopy and clathrin RNAi showed that DC-SIGN-mediated internalization occurs via clathrin-coated pits. Electron microscopy of ultrathin sections showed the involvement of DC-SIGN in clathrin-dependent HIV-1 internalization by DC. Currently, DC-specific C-type lectins are considered potential target in anti-tumor clinical trials. Detailed information about how different Ag are internalized via these receptors will facilitate the rational design of targeted therapeutic strategies.
This item appears in the following Collection(s)
- Academic publications [204996]
- Electronic publications [103294]
- Faculty of Medical Sciences [81051]
Upload full text
Use your RU credentials (u/z-number and password) to log in with SURFconext to upload a file for processing by the repository team.