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Publication year
2009Source
Journal of Oral Pathology & Medicine, 38, 8, (2009), pp. 630-8ISSN
Publication type
Article / Letter to editor

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Organization
Dentistry
Biochemistry (UMC)
Journal title
Journal of Oral Pathology & Medicine
Volume
vol. 38
Issue
iss. 8
Page start
p. 630
Page end
p. 8
Subject
NCEBP 2: Evaluation of complex medical interventions; NCMLS 3: Tissue engineering and pathologyAbstract
BACKGROUND: Wound contraction and scar formation after cleft palate repair impair the growth of the maxilla. The implantation of a growth factor-loaded scaffold might solve these problems. METHODS: The tissue response to fibroblast growth factor (FGF)-2 loaded collagen scaffolds was evaluated after implantation in the palate of rats. Scaffolds, with and without FGF-2, were implanted submucoperiosteally in the palate of 25 rats and evaluated after up to 16 weeks. On hematoxylin and eosin (H&E)-stained sections, the cell density and the number of giant cells within the scaffolds were quantified. Infiltration of inflammatory cells, myofibroblasts, and the number of blood vessels were quantified after immunohistochemistry. RESULTS: The cell density was significantly higher in the FGF-2 group up to 4 weeks after implantation (102% at 2 weeks, P < 0.001). The number of blood vessels was also significantly higher in the FGF-2 group at 1 and 2 weeks (316% at 1 week, P = 0.003), but the myofibroblast score was lower (100% at 2 weeks, P = 0.008). A comparable mild and rapidly subsiding inflammatory response and foreign body reaction were found in both groups. CONCLUSION: FGF-2-loaded scaffolds displayed a faster influx of host cells, an increased rate of vascularization, and a reduced differentiation of myofibroblasts. These scaffolds might therefore be highly suitable for intra-oral reconstructions, such as cleft palate repair.
This item appears in the following Collection(s)
- Academic publications [202606]
- Electronic publications [100803]
- Faculty of Medical Sciences [79948]
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