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Publication year
2009Source
Biochemical and Biophysical Research Communications, 382, 2, (2009), pp. 241-6ISSN
Publication type
Article / Letter to editor

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Organization
Cardiology
Journal title
Biochemical and Biophysical Research Communications
Volume
vol. 382
Issue
iss. 2
Page start
p. 241
Page end
p. 6
Subject
NCEBP 14: Cardiovascular diseasesAbstract
The purpose of this study was to study the effect of calcium, cyclic AMP (cAMP) and cyclic GMP (cGMP) on embryonic stem cell (ESC) motility during TNF-alpha-induced chemotaxis. ESCs were monitored using a chemotaxis chamber, with different concentrations of calcium or cAMP or cGMP added to the medium. Changes in intracellular calcium ([Ca(2+)](i)) were measured with the fluorescent dye fura-2/AM. We combined migratory parameters in a mathematical model and described it as "mobility". After adding calcium, a dose-dependant increase in cell speed was found. Cyclic AMP increased mobility as well as the [Ca(2+)](i). In contrast, adding dbcGMP resulted in a significant decrease in the mobility of the ESCs. During migration ESCs showed an increase in [Ca(2+)](i). Furthermore, TNF-alpha dramatically increased the movement as well as the directionality of ESCs. These results demonstrate that ESCs are highly motile and respond to different concentrations of calcium in a dose-related manner.
This item appears in the following Collection(s)
- Academic publications [227613]
- Electronic publications [107286]
- Faculty of Medical Sciences [86193]
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