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Title: Calcium and cyclic nucleotides affect TNF-alpha-induced stem cell migration.
Author(s): Kaplan, E. ; Min, J.Y.; Ke, Q.; Chen, Y.; Niethammer, M.; Rana, J.S.; Malek, S.; Verheugt, F.W.A. ; Morgan, J.P.
Publication year: 2009
Source: Biochemical and Biophysical Research Communications, vol. 382, iss. 2, (2009), pp. 241-246
ISSN: 0006-291X
DOI: https://doi.org/10.1016/j.bbrc.2009.02.068
Publication type: Article / Letter to editor

Please use this identifier to cite or link to this item : http://hdl.handle.net/2066/80403   http://hdl.handle.net/2066/80403

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Subject: NCEBP 14: Cardiovascular diseases
Organization: Cardiology
Journal title:
Biochemical and Biophysical Research Communications
Volume: vol. 382
Issue: iss. 2
Page start: p. 241
Page end: p. 246
Abstract:
The purpose of this study was to study the effect of calcium, cyclic AMP (cAMP) and cyclic GMP (cGMP) on embryonic stem cell (ESC) motility during TNF-alpha-induced chemotaxis. ESCs were monitored using a chemotaxis chamber, with different concentrations of calcium or cAMP or cGMP added to the medium. Changes in intracellular calcium ([Ca(2+)](i)) were measured with the fluorescent dye fura-2/AM. We combined migratory parameters in a mathematical model and described it as "mobility". After adding calcium, a dose-dependant increase in cell speed was found. Cyclic AMP increased mobility as well as the [Ca(2+)](i). In contrast, adding dbcGMP resulted in a significant decrease in the mobility of the ESCs. During migration ESCs showed an increase in [Ca(2+)](i). Furthermore, TNF-alpha dramatically increased the movement as well as the directionality of ESCs. These results demonstrate that ESCs are highly motile and respond to different concentrations of calcium in a dose-related manner.

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