Limited amounts of dendritic cells migrate into the T-cell area of lymph nodes but have high immune activating potential in melanoma patients.

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Publication year
2009Source
Clinical Cancer Research, 15, 7, (2009), pp. 2531-40ISSN
Publication type
Article / Letter to editor

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Organization
Tumorimmunology
Medical Oncology
Paediatrics - OUD tm 2017
Dermatology
Radiology
Pathology
Surgery
Laboratory of Medical Immunology
Nuclear Medicine
Journal title
Clinical Cancer Research
Volume
vol. 15
Issue
iss. 7
Page start
p. 2531
Page end
p. 40
Subject
N4i 4: Auto-immunity, transplantation and immunotherapy; NCMLS 2: Immune Regulation; NCMLS 6: Genetics and epigenetic pathways of disease; ONCOL 1: Hereditary cancer and cancer-related syndromes; ONCOL 2: Age-related aspects of cancer; ONCOL 3: Translational research; ONCOL 4: Quality of Care; ONCOL 5: Aetiology, screening and detectionAbstract
PURPOSE: The success of immunotherapy with dendritic cells (DC) to treat cancer is dependent on effective migration to the lymph nodes and subsequent activation of antigen-specific T cells. In this study, we investigated the fate of DC after intradermal (i.d.) or intranodal (i.n.) administration and the consequences for the immune activating potential of DC vaccines in melanoma patients. EXPERIMENTAL DESIGN: DC were i.d. or i.n. administered to 25 patients with metastatic melanoma scheduled for regional lymph node resection. To track DC in vivo with scintigraphic imaging and in lymph nodes by immunohistochemistry, cells were labeled with both [(111)In]-indium and superparamagnetic iron oxide. RESULTS: After i.d. injection, maximally 4% of the DC reached the draining lymph nodes. When correctly delivered, all DC were delivered to one or more lymph nodes after i.n. injection. Independent of the route of administration, large numbers of DC remained at the injection site, lost viability, and were cleared by infiltrating CD163+ macrophages within 48 hours. Interestingly, 87 +/- 10% of the surviving DC preferentially migrated into the T-cell areas, where they induced antigen-specific T-cell responses. Even though more DC reached the T-cell areas, i.n. injection of DC induced similar antigen-specific immune responses as i.d. injection. Immune responses were already induced with <5 x 10(5) DC migrating into the T-cell areas. CONCLUSIONS: Monocyte-derived DC have high immune activating potential irrespective of the route of vaccination. Limited numbers of DC in the draining lymph nodes are sufficient to induce antigen-specific immunologic responses.
This item appears in the following Collection(s)
- Academic publications [227864]
- Electronic publications [107337]
- Faculty of Medical Sciences [86218]
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