Advantages of indium-tin oxide-coated glass slides in correlative scanning electron microscopy applications of uncoated cultured cells.
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Publication year
2009Source
Journal of Microscopy, 233, 3, (2009), pp. 353-63ISSN
Publication type
Article / Letter to editor
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Organization
Cell Biology (UMC)
Journal title
Journal of Microscopy
Volume
vol. 233
Issue
iss. 3
Page start
p. 353
Page end
p. 63
Subject
IGMD 8: Mitochondrial medicine; NCMLS 4: Energy and redox metabolism; NCMLS 5: Membrane transport and intracellular motility; ONCOL 3: Translational researchAbstract
A method of direct visualization by correlative scanning electron microscopy (SEM) and fluorescence light microscopy of cell structures of tissue cultured cells grown on conductive glass slides is described. We show that by growing cells on indium-tin oxide (ITO)-coated glass slides, secondary electron (SE) and backscatter electron (BSE) images of uncoated cells can be obtained in high-vacuum SEM without charging artefacts. Interestingly, we observed that BSE imaging is influenced by both accelerating voltage and ITO coating thickness. By combining SE and BSE imaging with fluorescence light microscopy imaging, we were able to reveal detailed features of actin cytoskeletal and mitochondrial structures in mouse embryonic fibroblasts. We propose that the application of ITO glass as a substrate for cell culture can easily be extended and offers new opportunities for correlative light and electron microscopy studies of adherently growing cells.
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- Academic publications [245054]
- Electronic publications [132354]
- Faculty of Medical Sciences [93209]
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