
Fulltext:
71079.pdf
Embargo:
until further notice
Size:
507.1Kb
Format:
PDF
Description:
publisher's version
Publication year
2008Source
Journal of the American Chemical Society, 130, 9, (2008), pp. 2832-2841ISSN
Publication type
Article / Letter to editor

Display more detailsDisplay less details
Organization
Tumorimmunology
Journal title
Journal of the American Chemical Society
Volume
vol. 130
Issue
iss. 9
Page start
p. 2832
Page end
p. 2841
Subject
NCMLS 1: Immunity, infection and tissue repairAbstract
We report the design, synthesis, and biological testing of highly stable, nontoxic perfluoropolyether (PFPE) nanoemulsions for dual 19F MRI-fluorescence detection. A linear PFPE polymer was covalently conjugated to common fluorescent dyes (FITC, Alexa647 and BODIPy-TR), mixed with pluronic F68 and linear polyethyleneimine (PEI), and emulsified by microfluidization. Prepared nanoemulsions (<200 nm) were readily taken up by both phagocytic and non-phagocytic cells in vitro after a short (approximately 3 h) co-incubation. Following cell administration in vivo, 19F MRI selectively visualizes cell migration. Exemplary in vivo MRI images are presented of T cells labeled with a dual-mode nanoemulsion in a BALB/c mouse. Fluorescence detection enables fluorescent microscopy and FACS analysis of labeled cells, as demonstrated in several immune cell types including Jurkat cells, primary T cells and dendritic cells. The intracellular fluorescence signal is directly proportional to the 19F NMR signal and can be used to calibrate cell loading in vitro.
This item appears in the following Collection(s)
- Academic publications [204996]
- Electronic publications [103294]
- Faculty of Medical Sciences [81051]
Upload full text
Use your RU credentials (u/z-number and password) to log in with SURFconext to upload a file for processing by the repository team.