Simulated microgravity activates MAPK pathways in fibroblasts cultured on microgrooved surface topography.

Fulltext:
71030.pdf
Embargo:
until further notice
Size:
2.068Mb
Format:
PDF
Description:
publisher's version
Publication year
2008Source
Cell Motility and the Cytoskeleton, 65, 2, (2008), pp. 116-29ISSN
Publication type
Article / Letter to editor

Display more detailsDisplay less details
Organization
Periodontology and Biomaterials
Dentistry
Journal title
Cell Motility and the Cytoskeleton
Volume
vol. 65
Issue
iss. 2
Page start
p. 116
Page end
p. 29
Subject
NCMLS 1: Immunity, infection and tissue repair; NCMLS 3: Tissue engineering and pathologyAbstract
This study evaluated in vitro the differences in morphological behaviour between fibroblast cultured on smooth and microgrooved substrata (groove depth: 0.5 mum, width: 1 mum), which were subjected to simulated microgravity. The aim of the study was to clarify which of these parameters was more dominant to determine cell behaviour. Morphological characteristics were investigated using scanning electron microscopy and fluorescence microscopy in order to obtain qualitative information on cell alignment. Expression of collagen type I, and alpha1-, beta1-, beta3-integrin were investigated by QPCR. Finally, immunoblotting was applied to visualise MAPK signalling pathways. Microscopy and image analysis showed that the fibroblasts aligned along the groove direction on all textured surfaces. On the smooth substrata, cells had spread out in a random fashion. The alignment of cells cultured on grooved surfaces under simulated microgravity, after 48 h of culturing appeared similar to those cultured at 1g, although cell shape was different. Analysis of variance proved that all main parameters: topography, gravity force, and time were significant. In addition, gene levels were reduced by simulated microgravity particularly those of beta3-integrin and collagen, however alpha-1 and beta-1 integrin levels were up-regulated. ERK1/2 was reduced in RPM, however, JNK/SAPK and p38 remained active. The members of the small GTPases family were stimulated under microgravity, particularly RhoA and Cdc42. The results are in agreement that application of microgravity to fibroblasts promotes a change in their morphological appearance and their expression of cell-substratum proteins through the MAPK intracellular signalling pathways.
This item appears in the following Collection(s)
- Academic publications [227613]
- Electronic publications [107273]
- Faculty of Medical Sciences [86193]
Upload full text
Use your RU credentials (u/z-number and password) to log in with SURFconext to upload a file for processing by the repository team.