A biomaterial composed of collagen and solubilized elastin enhances angiogenesis and elastic fiber formation without calcification.
Publication year
2008Source
Tissue Engineering Part A, 14, 3, (2008), pp. 349-60ISSN
Publication type
Article / Letter to editor

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Organization
Biochemistry (UMC)
Pulmonary Diseases
Journal title
Tissue Engineering Part A
Volume
vol. 14
Issue
iss. 3
Page start
p. 349
Page end
p. 60
Subject
IGMD 9: Renal disorder; NCMLS 1: Immunity, infection and tissue repair; NCMLS 3: Tissue engineering and pathology; UMCN 4.1: Microbial pathogenesis and host defenseAbstract
Elastin is the prime protein in elastic tissues that contributes to elasticity of, for example, lung, aorta, and skin. Upon injury, elastic fibers are not readily replaced, which hampers tissue regeneration. Incorporation of solubilized elastin (hydrolyzed insoluble elastin fibers or elastin peptides) in biomaterials may improve regeneration, because solubilized elastin is able to promote proliferation as well as elastin synthesis. Porous biomaterials composed of highly purified collagen without and without elastin fibers or solubilized elastin were prepared by freezing and lyophilization. Solubilized elastin formed spherical structures that were incorporated in the collagenous part of the scaffolds and that persisted after chemical crosslinking of the scaffolds. Crosslinked scaffolds were subcutaneously implanted in young Sprague Dawley rats. Collagen-solubilized elastin and collagen scaffolds showed no calcification in this sensitive calcification model, in contrast to scaffolds containing elastin fibers. Collagen-solubilized elastin scaffolds also induced angiogenesis, as revealed by type IV collagen staining, and promoted elastic fiber synthesis, as shown with antibodies against rat elastin and fibrillin-1. It is concluded that scaffolds produced from collagen and solubilized elastin present a non-calcifying biomaterial with a capacity for soft-tissue regeneration, especially in relation to elastic fiber synthesis.
This item appears in the following Collection(s)
- Academic publications [204994]
- Electronic publications [103270]
- Faculty of Medical Sciences [81051]
- Open Access publications [71799]
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