Dynamic imaging of cancer growth and invasion: a modified skin-fold chamber model.
until further notice
SourceHistochemistry and Cell Biology, 130, 6, (2008), pp. 1147-54
Article / Letter to editor
Display more detailsDisplay less details
Cell Biology (UMC)
Histochemistry and Cell Biology
SubjectNCMLS 2: Immune Regulation; NCMLS 2: Metabolism, transport and motion; ONCOL 3: Translational research; UMCN 1.2: Molecular diagnosis, prognosis and monitoring
The metastatic invasion of cancer cells from the primary lesion into the adjacent stroma is a key step in cancer progression, and is associated with poor outcome. The principles of cancer invasion have been experimentally addressed in various in vitro models; however, key steps and mechanisms in vivo remain unclear. Here, we establish a modified skin-fold chamber model for orthotopic implantation, growth and invasion of human HT-1080 fibrosarcoma cells, dynamically reconstructed by epifluorescence and multiphoton microscopy. This strategy allows repeated imaging of tumor growth, tumor-induced angiogenesis and invasion, as either individual cells, or collective strands and cell masses that move along collagen-rich extracellular matrix and coopt host tissue including striated muscle strands and lymph vessels. This modified window model will be suited to address mechanisms of cancer invasion and metastasis, and related experimental therapy.
Upload full text
Use your RU credentials (u/z-number and password) to log in with SURFconext to upload a file for processing by the repository team.