Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes.

Fulltext:
70033.pdf
Embargo:
until further notice
Size:
1.149Mb
Format:
PDF
Description:
Publisher’s version
Publication year
2008Source
Proceedings of the National Academy of Sciences USA, 105, 11, (2008), pp. 4489-4494ISSN
Publication type
Article / Letter to editor

Display more detailsDisplay less details
Organization
Biochemistry (UMC)
Journal title
Proceedings of the National Academy of Sciences USA
Volume
vol. 105
Issue
iss. 11
Page start
p. 4489
Page end
p. 4494
Subject
NCMLS 1: Immunity, infection and tissue repair; NCMLS 7: Chemical and physical biology; ONCOL 3: Translational researchAbstract
Many aspects of plant development, including patterning and tropisms, are largely dependent on the asymmetric distribution of the plant signaling molecule auxin. Auxin transport inhibitors (ATIs), which interfere with directional auxin transport, have been essential tools in formulating this concept. However, despite the use of ATIs in plant research for many decades, the mechanism of ATI action has remained largely elusive. Using real-time live-cell microscopy, we show here that prominent ATIs such as 2,3,5-triiodobenzoic acid (TIBA) and 2-(1-pyrenoyl) benzoic acid (PBA) inhibit vesicle trafficking in plant, yeast, and mammalian cells. Effects on micropinocytosis, rab5-labeled endosomal motility at the periphery of HeLa cells and on fibroblast mobility indicate that ATIs influence actin cytoskeleton. Visualization of actin cytoskeleton dynamics in plants, yeast, and mammalian cells show that ATIs stabilize actin. Conversely, stabilizing actin by chemical or genetic means interferes with endocytosis, vesicle motility, auxin transport, and plant development, including auxin transport-dependent processes. Our results show that a class of ATIs act as actin stabilizers and advocate that actin-dependent trafficking of auxin transport components participates in the mechanism of auxin transport. These studies also provide an example of how the common eukaryotic process of actin-based vesicle motility can fulfill a plant-specific physiological role.
This item appears in the following Collection(s)
- Academic publications [202863]
- Electronic publications [100990]
- Faculty of Medical Sciences [80039]
Upload full text
Use your RU credentials (u/z-number and password) to log in with SURFconext to upload a file for processing by the repository team.