
Fulltext:
69712.pdf
Embargo:
until further notice
Size:
148.1Kb
Format:
PDF
Description:
Publisher’s version
Publication year
2008Author(s)
Number of pages
8 p.
Source
Birth Defects Research Part A-Clinical and Molecular Teratology, 82, 10, (2008), pp. 676-683ISSN
Publication type
Article / Letter to editor

Display more detailsDisplay less details
Organization
Paediatrics - OUD tm 2017
Biophysical Chemistry
Health Evidence
Internal Medicine
Endocrinology
Former Organization
Epidemiology, Biostatistics & HTA
Physical Chemistry/Biophysical Chemistry
Journal title
Birth Defects Research Part A-Clinical and Molecular Teratology
Volume
vol. 82
Issue
iss. 10
Page start
p. 676
Page end
p. 683
Subject
Biophysical Chemistry; EBP 1: Determinants in Health and Disease; IGMD 5: Health aging / healthy living; IGMD 6: Hormonal regulation; NCEBP 14: Cardiovascular diseases; NCEBP 1: Molecular epidemiology; NCMLS 4: Energy and redox metabolism; ONCOL 3: Translational research; UMCN 5.2: Endocrinology and reproductionAbstract
BACKGROUND: An impaired DNA methylation has been suggested to underlie the complex etiology of neural tube defects (NTDs). Previously, we have demonstrated that inhibition of methylation by periodate oxidized adenosine (Adox) results in a widening of the anterior neuropore (ANP) in our in vitro chick embryo model. Since DNA methylation is the chief regulator of gene expression, we hypothesize that inhibition of methylation by Adox in our in vitro chick embryo model will affect the expression of genes that may be involved in neurulation. In the present study, we therefore examined differential gene expression between Adox-treated and control chick embryos, using the Affymetrix Genechip Chicken Genome Array. METHODS: Chick embryos of 4/5 somites were cultured in vitro with saline (control) or Adox and cranial parts were excised. Gene expression profiling was determined using the Affymetrix Genechip Chicken Genome Array on RNA isolated from two pools of Adox-treated cranial parts (n = 12) and two pools of saline-treated cranial parts (n = 12). Microarray data were validated by QPCR analysis. RESULTS: In the Adox-treated chick embryos, 45 probesets were up-regulated (fold > or = 2.0, p < 0.05) and 32 probesets were down-regulated (fold < or = 0.5, p < 0.05). Of the 15 genes selected for QPCR analysis, the up-regulation of phosphoserine phosphatase (PSPH), unc-51-like kinase 1 (ULK1), and chemokine (C-X-C motif) ligand 12/stromal cell-derived factor 1 (CXCL12/SDF-1) was confirmed. CONCLUSIONS: Inhibition of methylation by Adox affects gene expression in our in vitro chick embryo model. Further research will focus on the gene-specific methylation patterns of PSPH, ULK1, and CXCL12/SDF-1 and the role of the products of these genes in neurulation.
This item appears in the following Collection(s)
- Academic publications [229037]
- Electronic publications [111437]
- Faculty of Medical Sciences [87745]
- Faculty of Science [34250]
Upload full text
Use your RU credentials (u/z-number and password) to log in with SURFconext to upload a file for processing by the repository team.