Clonal expansion of mutated mitochondrial DNA is associated with tumor formation and complex I deficiency in the benign renal oncocytoma.
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Publication year
2008Source
Human Molecular Genetics, 17, 7, (2008), pp. 986-95ISSN
Publication type
Article / Letter to editor
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Organization
Paediatrics - OUD tm 2017
Journal title
Human Molecular Genetics
Volume
vol. 17
Issue
iss. 7
Page start
p. 986
Page end
p. 95
Subject
IGMD 8: Mitochondrial medicine; NCMLS 4: Energy and redox metabolism; UMCN 5.3: Cellular energy metabolismAbstract
Mutations in mitochondrial DNA (mtDNA) are frequent in cancers but it is not yet clearly established whether they are modifier events involved in cancer progression or whether they are a consequence of tumorigenesis. Here we show a benign tumor type in which mtDNA mutations that lead to complex I (CI) enzyme deficiency are found in all tumors and are the only genetic alteration detected. Actually renal oncocytomas are homogeneous tumors characterized by dense accumulation of mitochondria and we had found that they are deficient in electron transport chain complex I (CI, NADH-ubiquinone oxidoreductase). In this work total sequencing of mtDNA showed that 9/9 tumors harbored point mutations in mtDNA, seven in CI genes, one in complex III, and one in the control region. 7/8 mutations were somatic. All tumors were somatically deficient for CI. The clonal amplification of mutated mtDNA in 8/9 tumors demonstrates that these alterations are selected and therefore favor or trigger growth. No nuclear DNA rearrangement was detected beside mtDNA defects. We hypothesize that functional deficiency of the oxidative phosphorylation CI could create a loop of amplification of mitochondria during cell division, impair substrates oxidation and increase intermediary metabolites availability.
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- Faculty of Medical Sciences [93461]
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