Development and characterization of enhanced green fluorescent protein and luciferase expressing cell line for non-destructive evaluation of tissue engineering constructs.
Publication year
2004Source
Biomaterials, 25, 27, (2004), pp. 5809-19ISSN
Publication type
Article / Letter to editor

Display more detailsDisplay less details
Organization
Periodontology and Biomaterials
Journal title
Biomaterials
Volume
vol. 25
Issue
iss. 27
Page start
p. 5809
Page end
p. 19
Subject
UMCN 4.3: Tissue engineering and reconstructive surgeryAbstract
This study investigates the utility of genetically modified cells developed for the qualitative and quantitative non-destructive evaluation of cells on biomaterials. The Fisher rat fibroblastic cell line has been genetically modified to stably express the reporter genes enhanced green fluorescence protein (EGFP) and luciferase. These reporter genes provide two unique opportunities to evaluate cell growth on materials without destruction of the sample. Utilizing the fluorescence of EGFP expressed in the cells, we were able to demonstrate distribution of cells in a oligo(poly(ethylene glycol) fumarate) hydrogel material and on a titanium fiber mesh scaffold using an inverted fluorescent light microscope. In addition, we were able to utilize a molecular light imaging system to macroscopically image the cells on these materials both with fluorescence and luminescence, as well as quantify the signal from the samples. Quantification of cell growth on the titanium mesh material for a period of 28 days was accomplished using the molecular light imaging system. Imaging was extended in vivo to cells on the titanium mesh scaffolds subcutaneously implanted in Fisher rats for a period of 28 days. This study outlines a non-destructive method to evaluate cells growing on biomaterials in vitro and in vivo.
This item appears in the following Collection(s)
- Academic publications [227693]
- Faculty of Medical Sciences [86198]
Upload full text
Use your RU credentials (u/z-number and password) to log in with SURFconext to upload a file for processing by the repository team.