Testis-specific human small heat shock protein HSPB9 is a cancer/testis antigen, and potentially interacts with the dynein subunit TCTEL1
Fulltext:
57197.pdf
Embargo:
until further notice
Size:
365.3Kb
Format:
PDF
Description:
Publisher’s version
Publication year
2004Source
European Journal of Cell Biology, 83, 7, (2004), pp. 337-45ISSN
Publication type
Article / Letter to editor
Display more detailsDisplay less details
Organization
Pathology
Biomolecular Chemistry
Journal title
European Journal of Cell Biology
Volume
vol. 83
Issue
iss. 7
Page start
p. 337
Page end
p. 45
Subject
Bio-Molecular Chemistry; UMCN 1.3: Tumor microenvironmentAbstract
Searching EST databases for new members of the human small heat shock protein family, we recently identified HSPB9, which is expressed exclusively in testis as determined by Northern blotting (Kappe et al., Biochim. Biophys. Acta 1520, 1-6, 2001). Here we confirm this testis-specific expression pattern by RT-PCR in a larger series of normal tissues. Interestingly, while screening HSPB9 ESTs, we also noted expression in tumours, which could be verified by RT-PCR. Protein expression of HSPB9 was also detected in normal human testis and various tumour samples using immunohistochemical staining. We thus conclude that HSPB9 belongs to the steadily growing number of cancer/testis antigens. To get a better understanding of the function of HSPB9, we performed a yeast two-hybrid screen to search for HSPB9-interacting proteins. TCTEL1, a light chain component of cytoplasmic and flagellar dynein, interacted in both the yeast two-hybrid system and in immunoprecipitation experiments with HSPB9. Additionally, immunohistochemical staining showed co-expression of HSPB9 and TCTEL1 in similar stages of spermatogenesis and in tumour cells. The possible functional significance of this interaction is discussed.
This item appears in the following Collection(s)
- Academic publications [246326]
- Electronic publications [133968]
- Faculty of Medical Sciences [93294]
- Faculty of Science [37964]
Upload full text
Use your RU credentials (u/z-number and password) to log in with SURFconext to upload a file for processing by the repository team.