Removal of heparan sulfate from the glomerular basement membrane blocks protein passage.
SourceJournal of the American Society of Nephrology, 18, 12, (2007), pp. 3119-27
Article / Letter to editor
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Paediatrics - OUD tm 2017
Journal of the American Society of Nephrology
SubjectDCN 1: Perception and Action; DCN 3: Neuroinformatics; IGMD 3: Genomic disorders and inherited multi-system disorders; IGMD 4: Glycostation disorders; IGMD 8: Mitochondrial medicine; IGMD 9: Renal disorder; N4i 4: Auto-immunity, transplantation and immunotherapy; NCMLS 1: Immunity, infection and tissue repair; NCMLS 1: Infection and autoimmunity; NCMLS 3: Tissue engineering and pathology; NCMLS 4: Energy and redox metabolism; ONCOL 3: Translational research; UMCN 3.1: Neuromuscular development and genetic disorders; UMCN 5.4: Renal disorders
Heparan sulfate (HS) within the glomerular basement membrane (GBM) is thought to play a major role in the charge-selective properties of the glomerular capillary wall. Recent data, however, raise questions regarding the direct role of HS in glomerular filtration. For example, in situ studies suggest that HS may prevent plasma macromolecules from clogging the GBM, keeping it in an "open" state. We evaluated this potential role of HS in vivo by studying the passage of protein through the glomerular capillary wall in the presence and absence of HS. Intravenous administration of neuraminidase removed neuraminic acid--but not HS--from the GBM, and this led to albuminuria. Concomitant removal of HS with heparinase III, confirmed by ultrastructural imaging, prevented the development of albuminuria in response to neuraminidase treatment. Taken together, these results suggest that HS keeps the GBM in an open state, facilitating passage of proteins through the glomerular capillary wall.
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