Lactococcus lactis GEM particles displaying pneumococcal antigens induce local and systemic immune responses following intranasal immunization.
Publication year
2006Source
Vaccine, 24, 26, (2006), pp. 5434-41ISSN
Publication type
Article / Letter to editor
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Organization
Paediatrics - OUD tm 2017
Journal title
Vaccine
Volume
vol. 24
Issue
iss. 26
Page start
p. 5434
Page end
p. 41
Subject
N4i 1: Pathogenesis and modulation of inflammation; NCMLS 1: Infection and autoimmunity; UMCN 4.1: Microbial pathogenesis and host defenseAbstract
The present work reports the use of non-living non-recombinant bacteria as a delivery system for mucosal vaccination. Antigens are bound to the cell-wall of pretreated Lactococcus lactis, designated as Gram-positive enhancer matrix (GEM), by means of a peptidoglycan binding domain. The influence of the GEM particles on the antigen-specific serum antibody response was studied. Following nasal immunization with the GEM-based vaccines, antibody responses were induced at systemic and local levels. Furthermore, different GEM-based vaccines could be used consecutively in the same mice without adverse effects or loss of activity. Taken together, the results evidence the adjuvant properties of the GEM particles and indicate that GEM-based vaccines can be used repeatedly and are particularly suitable for nasal immunization purposes.
This item appears in the following Collection(s)
- Academic publications [245131]
- Faculty of Medical Sciences [93207]
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