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Title: Expression and effect of inhibition of aminopeptidase-A during nephrogenesis.
Author(s): Dijkman, H.B.P.M. ; Assmann, K.J.M. ; Steenbergen, E. ; Wetzels, J.F.M.
Publication year: 2006
Source: Journal of Histochemistry and Cytochemistry, vol. 54, iss. 2, (2006), pp. 253-262
ISSN: 0022-1554
DOI: https://doi.org/10.1369/jhc.5A6815.2005
Publication type: Article / Letter to editor

Please use this identifier to cite or link to this item : https://hdl.handle.net/2066/49894   https://hdl.handle.net/2066/49894

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Subject: IGMD 7: Iron metabolism
IGMD 9: Renal disorder
NCMLS 1: Immunity, infection and tissue repair
UMCN 5.4: Renal disorders
Organization: Pathology
Nephrology
Journal title:
Journal of Histochemistry and Cytochemistry
Volume: vol. 54
Issue: iss. 2
Page start: p. 253
Page end: p. 262
Abstract:
Aminopeptidase-A (APA) is a metalloprotease that cleaves N-terminal aspartyl and glutamyl residues from peptides. Its best-known substrate is angiotensin II (Ang II), the most active compound of the renin-angiotensin system (RAS). The RAS is involved in renal development. Most components of the RAS system are expressed in the developing kidney. Thus far, APA has not been studied in detail. In the present study we have evaluated the expression of APA at the protein, mRNA, and enzyme activity (EA) level in the kidney during nephrogenesis. Furthermore, we have studied the effect of inhibiting APA EA by injection of anti-APA antibodies into 1-day-old mice. APA expression was observed from the comma stage onwards, predominantly in the developing podocytes and brush borders of proximal tubular cells. Notably, APA was absent in the medulla or the renal arterioles. Inhibition of APA EA caused temporary podocyte foot-process effacement, suggesting a minimum role for APA during nephrogenesis.

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