Inhibition of early steps in the lentiviral replication cycle by cathelicidin host defense peptides.
Publication year
2005Source
Retrovirology, 2, 1, (2005), pp. 2-1-2ISSN
Publication type
Article / Letter to editor

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Organization
Dermatology
Journal title
Retrovirology
Volume
vol. 2
Issue
iss. 1
Page start
p. 2-1
Page end
p. 2
Subject
UMCN 4.2: Chronic inflammation and autoimmunityAbstract
BACKGROUND: The antibacterial activity of host defense peptides (HDP) is largely mediated by permeabilization of bacterial membranes. The lipid membrane of enveloped viruses might also be a target of antimicrobial peptides. Therefore, we screened a panel of naturally occurring HDPs representing different classes for inhibition of early, Env-independent steps in the HIV replication cycle. A lentiviral vector-based screening assay was used to determine the inhibitory effect of HDPs on early steps in the replication cycle and on cell metabolism. RESULTS: Human LL37 and porcine Protegrin-1 specifically reduced lentiviral vector infectivity, whereas the reduction of luciferase activities observed at high concentrations of the other HDPs is primarily due to modulation of cellular activity and/ or cytotoxicity rather than antiviral activity. A retroviral vector was inhibited by LL37 and Protegrin-1 to similar extent, while no specific inhibition of adenoviral vector mediated gene transfer was observed. Specific inhibitory effects of Protegrin-1 were confirmed for wild type HIV-1. CONCLUSION: Although Protegrin-1 apparently inhibits an early step in the HIV-replication cycle, cytotoxic effects might limit its use as an antiviral agent unless the specificity for the virus can be improved.
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- Academic publications [227693]
- Electronic publications [107311]
- Faculty of Medical Sciences [86198]
- Open Access publications [76433]
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