Intercellular synchronization of intracellular calcium
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Publication year
2008Author(s)
Publisher
S.l. : s.n.,
ISBN
9789090228594
Number of pages
171 p.
Annotation
Radboud Universiteit Nijmegen, 08 mei 2008
Promotores : Gielen, C.C.A.M., Ypey, D.L.
Publication type
Dissertation
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Organization
Biophysics
Former Organization
Biophysics
Subject
BiophysicsAbstract
The aim of this thesis was to develop an integrated model that can combines an excitable membrane with an IP3-mediated intracellular calcium oscillator and which can explain the different growth-state dependent states of NRK-cells. Furthermore we investigated the interaction between electrically coupled NRK fibroblasts and propagation of action potentials and calcium waves through a network of NRK-cells using mathematical modeling. In Chapter 2 we present a single-cell model which is largely based on and supported by experimental data from normal rat kidney (NRK) fibroblasts. It will be shown that store dependent calcium (SDC)-channels play a stabilizing role in maintaining action-potential firing and intracellular calcium oscillations in NRK fibroblasts induced by external current pulses and/or increases in the intracellular IP3 concentration. In Chapter 3 we focus on the stability analysis of the model presented in Chapter 2. It shows how coupling of an excitable membrane with an intracellular calcium oscillator leads to a rich behavior of a cell with multiple stable and unstable states with hysteresis. In Chapter 4 it is examined how coupling of NRK cells by gap junctions leads to near-synchronization of intracellular calcium transients and propagation of action potentials in networks of NRK cells. Our general message is that calcium inflow through voltage dependent Ca-channels can reset the calcium oscillation mechanism and boost the propagation of electrical excitation. Finally, in Chapter 5 it is described under which conditions induction of local pacemaking activity in a network of gap junctionally coupled quiescent NRK fibroblasts may lead to a propagation of APs throughout the remainder of cells in the cellular network. Experimentally obtained results of calcium imaging of locally induced pacemaker activity in monolayers of quiescent NRK cells will be shown to correspond to the calculated behavior of a two-dimensional model of a network of NRK fibroblast in cell culture.
This item appears in the following Collection(s)
- Academic publications [245263]
- Dissertations [13779]
- Electronic publications [132514]
- Faculty of Science [37522]
- Open Access publications [106133]
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