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Publication year
2006Source
Journal of Controlled Release, 113, 1, (2006), pp. 63-72ISSN
Publication type
Article / Letter to editor

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Organization
Periodontology and Biomaterials
Nuclear Medicine
Synthetic Organic Chemistry
Physical Organic Chemistry
Former Organization
Physical Organic and Supramolecular Chemistry
Journal title
Journal of Controlled Release
Volume
vol. 113
Issue
iss. 1
Page start
p. 63
Page end
p. 72
Subject
N4i 1: Pathogenesis and modulation of inflammation; NCMLS 2: Immune Regulation; NCMLS 3: Tissue engineering and pathology; NCMLS 7: Chemical and physical biology; ONCOL 3: Translational research; ONCOL 5: Aetiology, screening and detection; Physical Organic Chemistry; UMCN 4.1: Microbial pathogenesis and host defense; UMCN 4.3: Tissue engineering and reconstructive surgeryAbstract
The focus of the present study was to functionalize multilayered DNA-coatings with the osteoinductive factor bone morphogenetic protein 2 (BMP-2) using different loading modalities. The multilayered DNA-coatings were built up from either poly-d-lysine (PDL) or poly(allylamine hydrochloride) (PAH) and DNA using electrostatic self-assembly (ESA). The amounts of BMP-2 loaded into the multilayered DNA-coatings and its subsequent release characteristics were determined using radiolabeled BMP-2. Additionally, the effect of BMP-2 functionalized multilayered DNA-coatings on the in vitro behavior of bone marrow-derived osteoblast-like cells was evaluated in terms of proliferation, differentiation, mineralization, and cell morphology. The results demonstrate the feasibility of multilayered DNA-coatings to be functionalized by embedding BMP-2 according to three different loading modalities: superficial (s), deep (d), and double-layer (dl). BMP-2 was incorporated proportionally into the multilayered DNA-coatings as: s+(4*d)=dl. All differently loaded multilayered DNA-coatings showed an initial burst release followed by an incremental sustained release of the remaining BMP-2. In vitro experiments demonstrated that the loaded factor remained biologically active, as an accelerated calcium deposition was observed on s- and dl-loaded multilayered DNA-coatings, without affecting cell proliferation. In contrast, d-loaded multilayered DNA-coatings influenced osteoblast-like cell behavior by decreasing the deposition of calcium.
This item appears in the following Collection(s)
- Academic publications [204994]
- Electronic publications [103280]
- Faculty of Medical Sciences [81051]
- Faculty of Science [32345]
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