Fabrication, characterization, and biological assessment of multilayered DNA-coatings for biomaterial purposes.

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Publication year
2006Source
Biomaterials, 27, 5, (2006), pp. 691-701ISSN
Publication type
Article / Letter to editor

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Organization
Periodontology and Biomaterials
Synthetic Organic Chemistry
Physical Organic Chemistry
Former Organization
Physical Organic and Supramolecular Chemistry
Journal title
Biomaterials
Volume
vol. 27
Issue
iss. 5
Page start
p. 691
Page end
p. 701
Subject
NCMLS 3: Tissue engineering and pathology; NCMLS 7: Chemical and physical biology; Physical Organic Chemistry; UMCN 4.3: Tissue engineering and reconstructive surgeryAbstract
This study describes the fabrication of two types of multilayered coatings onto titanium by electrostatic self-assembly (ESA), using deoxyribosenucleic acid (DNA) as the anionic polyelectrolyte and poly-d-lysine (PDL) or poly(allylamine hydrochloride) (PAH) as the cationic polyelectrolyte. Both coatings were characterized using UV-vis spectrophotometry, atomic force microscopy (AFM), X-ray photospectroscopy (XPS), contact angle measurements, Fourier transform infrared spectroscopy (FTIR), and for the amount of DNA immobilized. The mutagenicity of the constituents of the coatings was assessed. Titanium substrates with or without multilayered DNA-coatings were used in cell culture experiments to study cell proliferation, viability, and morphology. Results of UV-vis spectrophotometry, AFM, and contact angle measurements clearly indicated the progressive build-up of the multilayered coatings. Furthermore, AFM and XPS data showed a more uniform build-up and morphology of [PDL/DNA]-coatings compared to [PAH/DNA]-coatings. DNA-immobilization into both coatings was linear, and approximated 3microg/cm(2) into each double-layer. The surface morphology of both types of multilayered DNA-coatings showed elevations in the nanoscale range. No mutagenic effects of DNA, PDL, or PAH were detected, and cell viability and morphology were not affected by the presence of either type of multilayered DNA-coating. Still, the results of the proliferation assay revealed an increased proliferation of primary rat dermal fibroblasts on both types of multilayered DNA-coatings compared to non-coated controls. The biocompatibility and functionalization of the coatings produced here, will be assessed in subsequent cell culture and animal-implantation studies.
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- Academic publications [204996]
- Electronic publications [103294]
- Faculty of Medical Sciences [81051]
- Faculty of Science [32345]
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