Dietary modulation and structure prediction of rat mucosal pentraxin (Mptx) protein and loss of function in humans.
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Publication year
2007Source
Genes and Nutrition, 2, 3, (2007), pp. 275-285ISSN
Publication type
Article / Letter to editor
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Organization
CMBI
Bioinformatics
Journal title
Genes and Nutrition
Volume
vol. 2
Issue
iss. 3
Page start
p. 275
Page end
p. 285
Subject
Bioinformatics; UMCN 5.3: Cellular energy metabolismAbstract
Mucosal pentraxin (Mptx), identified in rats, is a short pentraxin of unknown function. Other subfamily members are Serum amyloid P component (SAP), C-reactive protein (CRP) and Jeltraxin. Rat Mptx mRNA is predominantly expressed in colon and in vivo is strongly (30-fold) regulated by dietary heme and calcium, modulators of colon cancer risk. This renders Mptx a potential nutrient sensitive biomarker of gut health. To support a role as biomarker, we examined whether the pentraxin protein structure is conserved, whether Mptx protein is nutrient-sensitively expressed and whether Mptx is expressed in mouse and human. Sequence comparison and 3D modelling showed that rat Mptx is highly homologous to the other pentraxins. The calcium-binding site and subunit interaction sites are highly conserved, while a loop deletion and charged residues contribute to a distinctive �top� face of the pentamer. In accordance with mRNA expression, Mptx protein is strongly down-regulated in rat colon mucosa in response to high dietary heme intake. Mptx mRNA is expressed in rat and mouse colon, but not in human colon. A stop codon at the beginning of human exon two indicates loss of function, which may be related to differences in intestinal cell turnover between man and rodents.
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- Faculty of Medical Sciences [93208]
- Faculty of Science [37522]
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