Expression and physiological regulation of BDNF receptors in the neuroendocrine melanotrope cell of Xenopus laevis

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Title:	Expression and physiological regulation of BDNF receptors in the neuroendocrine melanotrope cell of Xenopus laevis
Author(s):	Kidane, A.H. ; van Dooren, S.H.; Roubos, E.W. ; Jenks, B.G.
Publication year:	2007
Source:	General and Comparative Endocrinology, vol. 153, iss. 1-3, (2007), pp. 176-181
ISSN:	0016-6480
Related links:	http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17502112
DOI:	https://doi.org/10.1016/j.ygcen.2007.04.001
Publication type:	Article / Letter to editor
Please use this identifier to cite or link to this item : https://hdl.handle.net/2066/34773
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Subject:	Neurophysiology
Organization:	Neurophysiology
Former Organization:	Cellular Animal Physiology
Journal title:	General and Comparative Endocrinology
Volume:	vol. 153
Issue:	iss. 1-3
Page start:	p. 176
Page end:	p. 181
Abstract:	Brain-derived neurotrophic factor (BDNF) and alpha-melanophore-stimulating hormone (alpha-MSH) are co-sequestered in secretory granules in melanotrope cells of the pituitary pars intermedia of the amphibian Xenopus laevis. alpha-MSH is responsible for pigment dispersion in dermal melanophores during the process of black-background adaptation. BDNF-production in melanotrope cells is increased by placing animals on a black background, and BDNF acts as an autocrine stimulatory factor on the melanotrope cells. However, the repertoire of possible neurotrophin receptors of the melanotrope is unknown. In this study we have established the expression of full length TrkB (TrkB.FL), truncated TrkB (TrkB.T) and p75(NTR) receptors in the Xenopus neurointermediate lobe by RT-PCR. In situ hybridization reveals the presence of TrkB.FL mRNA and p75(NTR) mRNA in melanotrope cells. Quantitative RT-PCR shows that in animals on a black background the amounts of TrkB.T and p75(NTR) mRNA are about three times higher than in white background-adapted animals. We suggest that the amount of p75(NTR) sets the sensitivity of the melanotrope cells for the stimulatory action of BDNF during physiological adaptation to background light intensity.