Impact of secreted glucanases upon the cell surface and fitness of Candida albicans during colonisation and infection.
Publication year
2024Source
The Cell Surface, 11, (2024), pp. 100128, article 100128ISSN
Annotation
01 juni 2024
Publication type
Article / Letter to editor
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Organization
Internal Medicine
Journal title
The Cell Surface
Volume
vol. 11
Page start
p. 100128
Subject
Internal Medicine - Radboud University Medical CenterAbstract
Host recognition of the pathogen-associated molecular pattern (PAMP), β-1,3-glucan, plays a major role in antifungal immunity. β-1,3-glucan is an essential component of the inner cell wall of the opportunistic pathogen Candida albicans. Most β-1,3-glucan is shielded by the outer cell wall layer of mannan fibrils, but some can become exposed at the cell surface. In response to host signals such as lactate, C. albicans shaves the exposed β-1,3-glucan from its cell surface, thereby reducing the ability of innate immune cells to recognise and kill the fungus. We have used sets of barcoded xog1 and eng1 mutants to compare the impacts of the secreted β-glucanases Xog1 and Eng1 upon C. albicans in vitro and in vivo. Flow cytometry of Fc-dectin-1-stained strains revealed that Eng1 plays the greater role in lactate-induced β-1,3-glucan masking. Transmission electron microscopy and stress assays showed that neither Eng1 nor Xog1 are essential for cell wall maintenance, but the inactivation of either enzyme compromised fungal adhesion to gut and vaginal epithelial cells. Competitive barcode sequencing suggested that neither Eng1 nor Xog1 strongly influence C. albicans fitness during systemic infection or vaginal colonisation in mice. However, the deletion of XOG1 enhanced C. albicans fitness during gut colonisation. We conclude that both Eng1 and Xog1 exert subtle effects on the C. albicans cell surface that influence fungal adhesion to host cells and that affect fungal colonisation in certain host niches.
This item appears in the following Collection(s)
- Academic publications [243984]
- Electronic publications [130873]
- Faculty of Medical Sciences [92811]
- Open Access publications [105042]
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