AlbuMAX supplemented media induces the formation of transmission-competent P. falciparum gametocytes.
Publication year
2024Source
Molecular and Biochemical Parasitology, 259, (2024), pp. 111634, article 111634ISSN
Annotation
01 september 2024
Publication type
Article / Letter to editor
![https://hdl.handle.net/2066/307517](/themes/Mirage2//images/copy.png)
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Organization
Medical Microbiology
IQ health
Journal title
Molecular and Biochemical Parasitology
Volume
vol. 259
Page start
p. 111634
Subject
IQ health - Radboud University Medical Center; Medical Microbiology - Radboud University Medical CenterAbstract
Asexual blood stage culture of Plasmodium falciparum is routinely performed but reproducibly inducing commitment to and maturation of viable gametocytes remains difficult. Culture media can be supplemented with human serum substitutes to induce commitment but these generally only allow for long-term culture of asexual parasites and not transmission-competent gametocytes due to their different lipid composition. Recent insights demonstrated the important roles lipids play in sexual commitment; elaborating on this we exposed ring stage parasites (20-24 hours hpi) for one day to AlbuMAX supplemented media to trigger induction to gametocytogenesis. We observed a significant increase in gametocytes after AlbuMAX induction compared to serum. We also tested the transmission potential of AlbuMAX inducted gametocytes and found a significant higher oocyst intensity compared to serum. We conclude that AlbuMAX supplemented media induces commitment, allows a more stable and predictable production of transmittable gametocytes than serum alone.
This item appears in the following Collection(s)
- Academic publications [244127]
- Electronic publications [131133]
- Faculty of Medical Sciences [92874]
- Open Access publications [105172]
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