Characterization of myelodysplastic syndromes hematopoietic stem and progenitor cells using mass cytometry.
Publication year
2023Source
Cytometry. Part B-Clinical Cytometry, 104, 2, (2023), pp. 128-140ISSN
Publication type
Article / Letter to editor
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Organization
Laboratory Medicine
Journal title
Cytometry. Part B-Clinical Cytometry
Volume
vol. 104
Issue
iss. 2
Page start
p. 128
Page end
p. 140
Subject
Radboudumc 2: Cancer development and immune defence Laboratory Medicine; Radboud University Medical CenterAbstract
BACKGROUND: Myelodysplastic syndromes (MDS) at risk of transformation to acute myeloid leukemia (AML) are difficult to identify. The bone marrows of MDS patients harbor specific hematopoietic stem and progenitor cell (HSPC) abnormalities that may be associated with sub-types and risk-groups. Leukemia-associated characteristics of such cells may identify MDS patients at risk of progression to AML and provide insight in the pathobiology of MDS. METHODS: Bone marrow samples from healthy donors (n = 10), low risk (n = 12) and high risk (n = 13) MDS patients were collected, in addition, AML samples for 5 out of 6 MDS patients that progressed. Mass cytometry was applied to assess expression of stem cell subset and leukemia-associated immunophenotype markers. RESULTS: We analyzed the data using FlowSOM to cluster cells with similar expression of 10 commonly used stem cell markers. Metaclusters (n = 20) of these clusters represented populations of cells with a related phenotype, largely resembling known stem cell subsets. Within specific subsets, intra-cellular expression levels of pCREB, IkBα, or pS6 differed significantly between healthy bone marrow (HBM) and MDS or consecutive secondary AML samples. CD34, CD44, and CD49f expression was significantly increased in high risk MDS and AML-associated metaclusters. We identified MDS/sAML cells with aberrant phenotypes when compared to HBM. Such cells were observed in clusters of both primary MDS and secondary AML samples. CONCLUSIONS: High-dimensional mass cytometry and computational data analyses enabled characterization of HSPC subsets in MDS and identification of leukemia stem cell populations based on their immunophenotype. Stem cells in MDS that display leukemia-associated features may predict the risk of developing AML.
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- Academic publications [245263]
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- Faculty of Medical Sciences [93208]
- Open Access publications [106157]
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