Expression of the novel epithelial Ca2+ channel ECaC1 in rat pancreatic islets.
SourceJournal of Histochemistry and Cytochemistry, 50, 6, (2002), pp. 789-98
Article / Letter to editor
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Molecular Animal Physiology
Journal of Histochemistry and Cytochemistry
SubjectRegulation of salt and water reabsorption in the renal collecting duct; Chemical Endocrinology; Endocrinology; Regulatie water en zouttransport in de verzamelbuis van de nier
The epithelial Ca2+ channel, ECaC1, is primarily expressed in the apical membrane of vitamin D-responsive tissues. This study characterizes for the first time the presence of this novel channel in pancreatic tissue by reverse transcriptase-polymerase chain reaction and immunohistochemistry. In addition, the expression of ECaC1 was investigated in an animal model for Type 2 diabetes mellitus, the Zucker diabetic fatty (ZDF) rat. Identical staining patterns for ECaC1 and insulin were observed, whereas no co-localization of ECaC1 with glucagon was found. ECaC1, insulin, and prohormone convertase 1 (a neuroendocrine endoprotease expressed in secretory granules) showed a similar punctate staining. ECaC1 co-localized with the Ca2+ binding protein calbindin-D(28K) in the beta-cells. Furthermore, in contrast to wild-type rats, in ZDF rats aging led to a progressive decrease in both insulin and ECaC1 staining. Plasma 1,25-dihydroxyvitamin D3 levels were similar in both control and ZDF rats and decreased with aging. Taken together, our findings indicate that this novel Ca2+ channel may play a role in the regulation of endocrine Ca2+ homeostasis.
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