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      Expanding the verrucomicrobial methanotrophic world: Draft genomes of two novel species of Methylacidimicrobium gen. nov.

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      Creators
      Cremers, G.
      Pol, A.
      Jetten, M.S.M.
      Camp, H.J.M. op den
      Date of Archiving
      2019
      Archive
      European Nucleotide Archive
      Related links
      https://www.ebi.ac.uk/ena/browser/view/PRJEB32513
      Related publications
      Draft Genome Sequences of Two Acidophilic, Mesophilic Verrucomicrobial Methanotrophs Contain Only One pmoCAB Operon  
      Publication type
      Dataset
      Access level
      Open access
      Please use this identifier to cite or link to this item: https://hdl.handle.net/2066/239546   https://hdl.handle.net/2066/239546
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      Organization
      Ecological Microbiology
      Audience(s)
      Biology
      Key words
      Sequence; Acidophilic; Mesophilic; Verrucomicrobe; Methanotroph; pmoCAB Operon
      Abstract
      Methanotrophic Verrucomicrobia have been found in geothermal environments characterized by high temperatures and low pH values. We have isolated and characterized two new species of mesophilic acidophilic verrucomicrobial methanotrophs from a volcanic soil in Italy. On basis of their 16S rRNA phylogeny we proposed the new genus Methylacidimicrobium. The draft genomes of Methylacidimicrobium tartarophylax 4AC (2.75 Mb) and Methylacidimicrobium cyclophantes 3B (2.44 Mb) have a GC content of 60.9% and encode for 2,804 and 2,511 ORFs, respectively. The metabolic machinery was similar to that seen with thermophilic verucomicrobial methanotrophs (genus Methylacidiphilum). The two genomes show only one complete pmoCAB operon but two different xoxF genes encoding the XoxF-type lanthanide-dependent methanol dehydrogenases. The latter genes possess the typical coordination-specific aspartate located two amino acids downstream of the catalytic aspartate. In addition all genes involved in the Calvin cycle, including the two subunits of the key enzyme RuBisCO, were present.
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      • Faculty of Science [33762]
       
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