Anammox enrichment culture glycoproteomics
Date of Archiving2021
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Key wordswastewater; metagenome; anammox reactor; Brocadia sapporoensis
The physiology of the planctomycetal anammox bacteria makes them particularly special because they share features with all three domains of life. Anammox bacteria have been reported recently to produce surface-layer proteins, which represent the outermost layer and provide structure, shape and protection under extreme conditions. Furthermore, we report on the unique cell surface-layer glycosylatio... Read more Sample Processing Protocol SAMPLE PREPARATION. Biomass from enrichment cultures and reference strains were transferred into a B-PER/TEAB buffer solution (50mM TEAB, 1% (w/w) NaDOC, adjusted to pH 8.0, incl. protease inhibitor) and lysed using bead-beating homogenization. In the following, the homogenate were centrifuged and the supernatant was transferred to a new tube before precipitating the protein content using TCA (tri... Read more Data Processing Protocol Whole cell lysate shotgun proteomics raw data were analysed using PEAKS Studio X (Bioinformatics Solutions Inc., Canada). The anammox enrichment cultures were searched against a metagenomics constructed database (two-round search, to create a focused database in the initial search), where the pure reference strains were analysed using a Uniprot reference proteome database (of closely related subst...
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