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Publication year
2020Author(s)
Source
Nature Nanotechnology, 15, 5, (2020), pp. 398-405ISSN
Publication type
Article / Letter to editor
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Organization
Nephrology
Journal title
Nature Nanotechnology
Volume
vol. 15
Issue
iss. 5
Page start
p. 398
Page end
p. 405
Subject
Radboudumc 11: Renal disorders RIMLS: Radboud Institute for Molecular Life SciencesAbstract
Ischaemic heart disease evokes a complex immune response. However, tools to track the systemic behaviour and dynamics of leukocytes non-invasively in vivo are lacking. Here, we present a multimodal hot-spot imaging approach using an innovative high-density lipoprotein-derived nanotracer with a perfluoro-crown ether payload ((19)F-HDL) to allow myeloid cell tracking by (19)F magnetic resonance imaging. The (19)F-HDL nanotracer can additionally be labelled with zirconium-89 and fluorophores to detect myeloid cells by in vivo positron emission tomography imaging and optical modalities, respectively. Using our nanotracer in atherosclerotic mice with myocardial infarction, we observed rapid myeloid cell egress from the spleen and bone marrow by in vivo (19)F-HDL magnetic resonance imaging. Concurrently, using ex vivo techniques, we showed that circulating pro-inflammatory myeloid cells accumulated in atherosclerotic plaques and at the myocardial infarct site. Our multimodality imaging approach is a valuable addition to the immunology toolbox, enabling the study of complex myeloid cell behaviour dynamically.
This item appears in the following Collection(s)
- Academic publications [238441]
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- Faculty of Medical Sciences [90373]
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