Publication year
2001Source
Nucleosides, Nucleotides and Nucleic Acids, 20, 4-7, (2001), pp. 1271-1274ISSN
Publication type
Article / Letter to editor

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Organization
Public Health
Journal title
Nucleosides, Nucleotides and Nucleic Acids
Volume
vol. 20
Issue
iss. 4-7
Page start
p. 1271
Page end
p. 1274
Subject
Public HealthAbstract
A new facile method for spin-labeling suitable for DNA and RNA oligonucleotides is presented. The nitroxide 3-ethenyl-2,2,5,5-tetramethyl-pyrrolin-1-yloxy was directly introduced during automated solid-phase synthesis by a Pd(0) cross coupling reaction. The main advantages of this procedure are the small amount of spin-label needed for the derivatisation of the oligonucleotide and the high coupling efficiency on the solid phase.
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