Complete sequence analysis of the A*1103 allele.
SourceTissue Antigens, 55, (2000), pp. 68-70
Article / Letter to editor
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Blood Transfusion and Transplantation Immunology
SubjectDetection and characterization of new HLA class I and class II variants by DNA based typing techniques and direct sequencing.; Clinical description and delineation of genetic syndromes; Detectie en karakterisering van nieuwe HLA klasse I en II varianten.; Klinische beschrijving en moleculaire definiëring van genetische syndromen
Here we report the full-length sequence of a novel A*11 variant. The variant was identified by ARMS-PCR and serology, the sequence was confirmed by cloning and subsequent sequencing. This variant, A*1103, found in a family of oriental origin, resembles the A*1101 sequence in exon 2 but differs in exon 3 with regard to codons 151 and 152. The polymorphism's result in two amino acid substitutions (one conserved (His->Arg), one introducing a negative charge (Ala>Glu)) located in the alpha 2 helical region. The arginine at amino acid position 151 is rare amongst A-locus alleles and is besides A*1103 only observed in A*29 variants, the glutamine at amino acid position 152 is shared with A*0301, A*25, *26, *34 variants and the A*02 subtypes subtypes A*0203, *0213 and *0226. In fact, the amino acid motif comprising codons 151 and 152 is unique to A*1103 among A-locus alleles, but is common to C-locus alleles
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