An assay system to detect the selective advantage of anti-HIV ribozyme expressing CD4+ T-lymphocytes.

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Title:	An assay system to detect the selective advantage of anti-HIV ribozyme expressing CD4+ T-lymphocytes.
Author(s):	Klein, S.A.; Klebba, C.; Engels, J.A.
Publication year:	2001
Source:	Nucleosides, Nucleotides and Nucleic Acids, vol. 20, iss. 4-7, (2001), pp. 629--34
ISSN:	1525-7770
DOI:	http://dx.doi.org/10.1081/NCN-100002339
Publication type:	Article / Letter to editor
Please use this identifier to cite or link to this item : http://hdl.handle.net/2066/185563
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Subject:	Public Health
Organization:	Public Health
Journal title:	Nucleosides, Nucleotides and Nucleic Acids
Volume:	vol. 20
Issue:	iss. 4-7
Page start:	p. 629-
Page end:	p. 34
Abstract:	This study was undertaken to establish an assay system to detect the survival advantage of anti-HIV ribozyme expressing cells under the selective pressure of HIV infection. In a mixture with wild type cells the proportion of ribozyme expressing cells was increased up to 12-fold. As a mechanism of the selective advantage an inhibition of HIV induced apoptotic cell death could be demonstrated. Furthermore, a dose dependency of the antiviral ribozyme effects was observed.