Novel targeting strategy for generating mouse models with defects in the retinoid cycle.
SourceVision Research, 43, 28, (2003), pp. 3075-3079
Article / Letter to editor
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SubjectUMCN 3.3: Neurosensory disorders; UMCN 5.3: Cellular energy metabolism
In addition to RDH5, other enzymes capable of oxidizing 11-cis-retinol are present within the retinal pigment epithelium, Muller cells and/or photoreceptors. Candidate proteins have meanwhile been identified. To study the physiological and pathological aspects of these enzymes, mice in which these genes are no longer functional are being generated. A fast-targeting strategy for the disruption of genes was developed. Generation of double and triple knockouts will aid in determining if these retinol dehydrogenases are responsible for the remaining 11-cis-retinol oxidation observed in RDH5 knockout animals.
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