Consensus strategy to quantitate malignant cells in myeloma patients is validated in a multicenter study.
Publication year
2000Source
Blood, 96, 1, (2000), pp. 63-70ISSN
Publication type
Article / Letter to editor
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Organization
Haematology
Internal Medicine
CHL
Journal title
Blood
Volume
vol. 96
Issue
iss. 1
Page start
p. 63
Page end
p. 70
Subject
Molecular pathogenesis of multiple myeloma; Moleculaire pathogenese van het multipele myeloom loom (MM)Abstract
Recently the Belgium-dutch Hematology-Oncology group installed a multicenter study to evaluate a multicenter study to wether myeloma patients treated with intensive chemotherapy benefit rom additional perpheral stem cell transplantation. To determine treatment response accurately, we decided to quantitate malignant cells. To test a consensus qunatitation strategy, 5 centers independently determined the immunoglobulin heavy chain sequences of patient tumor cells and developed allele-specific oligonucleotides (ASO) and ASO-polymerase chain reaction (PCR). We compared the reproducibility of realtime quantitation with quantitation using limiting dilutions (4 centers), respectively. Within single centers we found an average variation coefficient of 0.74 with limiting dilutions not significantly different from the average 0.82 center-t0-center variation coefficient. Within a single center. real-time quantitation proved more reproducible (average variation coefficient 0.36). Quantification was comfirmed in 3 patients durign treatment in the protocol. This rep[ort shows that real-time PCR or limiting dilution assays can be used for quantitation in a single multicenter trial. We present consensus strategy that allows an accurate comparison of quantitation data genereted in independent centers.
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- Faculty of Medical Sciences [90359]
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