Role of Vitamin D in Maintaining Renal Epithelial Barrier Function in Uremic Conditions
Publication year
2017Source
International Journal of Molecular Sciences, 18, 12, (2017), pp. 2531, article 2531ISSN
Publication type
Article / Letter to editor
Display more detailsDisplay less details
Organization
Nephrology
Pharmacology-Toxicology
Radboudumc Extern
Journal title
International Journal of Molecular Sciences
Volume
vol. 18
Issue
iss. 12
Page start
p. 2531
Subject
Radboudumc 11: Renal disorders RIHS: Radboud Institute for Health Sciences; Radboudumc 11: Renal disorders RIMLS: Radboud Institute for Molecular Life SciencesAbstract
As current kidney replacement therapies are not efficient enough for end-stage renal disease (ESRD) treatment, a bioartificial kidney (BAK) device, based on conditionally immortalized human proximal tubule epithelial cells (ciPTEC), could represent an attractive solution. The active transport activity of such a system was recently demonstrated. In addition, endocrine functions of the cells, such as vitamin D activation, are relevant. The organic anion transporter 1 (OAT-1) overexpressing ciPTEC line presented 1alpha-hydroxylase (CYP27B1), 24-hydroxylase (CYP24A1) and vitamin D receptor (VDR), responsible for vitamin D activation, degradation and function, respectively. The ability to produce and secrete 1alpha,25-dihydroxy-vitamin D(3), was shown after incubation with the precursor, 25-hydroxy-vitamin D(3). The beneficial effect of vitamin D on cell function and behavior in uremic conditions was studied in the presence of an anionic uremic toxins mixture. Vitamin D could restore cell viability, and inflammatory and oxidative status, as shown by cell metabolic activity, interleukin-6 (IL-6) levels and reactive oxygen species (ROS) production, respectively. Finally, vitamin D restored transepithelial barrier function, as evidenced by decreased inulin-FITC leakage in biofunctionalized hollow fiber membranes (HFM) carrying ciPTEC-OAT1. In conclusion, the protective effects of vitamin D in uremic conditions and proven ciPTEC-OAT1 endocrine function encourage the use of these cells for BAK application.
This item appears in the following Collection(s)
- Academic publications [238441]
- Electronic publications [122523]
- Faculty of Medical Sciences [90373]
- Open Access publications [97518]
Upload full text
Use your RU credentials (u/z-number and password) to log in with SURFconext to upload a file for processing by the repository team.