Controlled release of antigen and Toll-like receptor ligands from PLGA nanoparticles enhances immunogenicity
Publication year
2017Source
Nanomedicine, 12, 5, (2017), pp. 491-510ISSN
Publication type
Article / Letter to editor
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Organization
Tumorimmunology
Journal title
Nanomedicine
Volume
vol. 12
Issue
iss. 5
Page start
p. 491
Page end
p. 510
Subject
Radboudumc 17: Women's cancers RIMLS: Radboud Institute for Molecular Life Sciences; Radboudumc 2: Cancer development and immune defence RIMLS: Radboud Institute for Molecular Life SciencesAbstract
AIM: Dendritic cells rapidly capture nanoparticles and induce a potent cellular immune response. It is yet unknown whether the immunological response induced by slow release of encapsulated versus soluble antigen and adjuvant is superior. MATERIALS & METHODS: The kinetics of poly(lactic-co-glycolic acid) PLGA nanoparticles antigen release was studied by the DQ-bovine serum albumin (BSA) self-quenching antigen model. The immunological response induced was evaluated by means of dendritic cell activation/maturation markers, cytokine production and their ability to drive antigen-specific T-cell proliferation. RESULTS & CONCLUSION: PLGA-encapsulated antigen and adjuvant showed an enhanced T-cell response when compared with soluble vaccine components by increasing antigenicity and adjuvanticity. Although the kinetic profile followed the same pattern, encapsulation increased strength and duration of the response.
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- Academic publications [238441]
- Faculty of Medical Sciences [90373]
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