Limitations of the hCMEC/D3 cell line as a model for Abeta clearance by the human blood-brain barrier
Publication year
2017Source
Journal of Neuroscience Research, 95, 7, (2017), pp. 1513-1522ISSN
Publication type
Article / Letter to editor

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Organization
Neurology
Pathology
Laboratory Medicine
Journal title
Journal of Neuroscience Research
Volume
vol. 95
Issue
iss. 7
Page start
p. 1513
Page end
p. 1522
Subject
Radboudumc 1: Alzheimer`s disease DCMN: Donders Center for Medical Neuroscience; Radboudumc 3: Disorders of movement DCMN: Donders Center for Medical NeuroscienceAbstract
Alzheimer's disease and cerebral amyloid angiopathy are characterized by accumulation of amyloid-beta (Abeta) at the cerebrovasculature due to decreased clearance at the blood-brain barrier (BBB). However, the exact mechanism of Abeta clearance across this barrier has not been fully elucidated. The hCMEC/D3 cell line has been characterized as a valid model for the BBB. In this study we evaluated the use of this model to study Abeta clearance across the BBB, with an emphasis on brain-to-blood directional permeability. Barrier integrity of hCMEC/D3 monolayers was confirmed for large molecules in both the apical to basolateral and the reverse direction. However, permeability for smaller molecules was substantially higher, especially in basolateral to apical direction, and barrier formation for Abeta was completely absent in this direction. In addition, hCMEC/D3 cells failed to develop a high TEER, possibly caused by incomplete formation of tight junctions. We conclude that the hCMEC/D3 model has several limitations to study the cerebral clearance of Abeta. Therefore, the model needs further characterization before this cell system can be generally applied as a model to study cerebral Abeta clearance. (c) 2016 The Authors Journal of Neuroscience Research Published by Wiley Periodicals, Inc.
This item appears in the following Collection(s)
- Academic publications [202802]
- Electronic publications [100870]
- Faculty of Medical Sciences [80020]
- Open Access publications [69592]
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