Impact of volunteer-related and methodology-related factors on the reproducibility of brachial artery flow-mediated vasodilation: analysis of 672 individual repeated measurements.
Publication year
2016Source
Journal of Hypertension, 34, 9, (2016), pp. 1738-45ISSN
Annotation
01 september 2016
Publication type
Article / Letter to editor
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Organization
Physiology
Journal title
Journal of Hypertension
Volume
vol. 34
Issue
iss. 9
Page start
p. 1738
Page end
p. 45
Subject
Radboudumc 16: Vascular damage RIHS: Radboud Institute for Health Sciences; Radboudumc 6: Metabolic Disorders RIHS: Radboud Institute for Health SciencesAbstract
OBJECTIVES: Brachial artery flow-mediated dilation (FMD) is a popular technique to examine endothelial function in humans. Identifying volunteer and methodological factors related to variation in FMD is important to improve measurement accuracy and applicability. METHODS: Volunteer-related and methodology-related parameters were collected in 672 volunteers from eight affiliated centres worldwide who underwent repeated measures of FMD. All centres adopted contemporary expert-consensus guidelines for FMD assessment. After calculating the coefficient of variation (%) of the FMD for each individual, we constructed quartiles (n = 168 per quartile). Based on two regression models (volunteer-related factors and methodology-related factors), statistically significant components of these two models were added to a final regression model (calculated as beta-coefficient and R). This allowed us to identify factors that independently contributed to the variation in FMD%. RESULTS: Median coefficient of variation was 17.5%, with healthy volunteers demonstrating a coefficient of variation 9.3%. Regression models revealed age (beta = 0.248, P < 0.001), hypertension (beta = 0.104, P < 0.001), dyslipidemia (beta = 0.331, P < 0.001), time between measurements (beta = 0.318, P < 0.001), lab experience (beta = -0.133, P < 0.001) and baseline FMD% (beta = 0.082, P < 0.05) as contributors to the coefficient of variation. After including all significant factors in the final model, we found that time between measurements, hypertension, baseline FMD% and lab experience with FMD independently predicted brachial artery variability (total R = 0.202). CONCLUSION: Although FMD% showed good reproducibility, larger variation was observed in conditions with longer time between measurements, hypertension, less experience and lower baseline FMD%. Accounting for these factors may improve FMD% variability.
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- Academic publications [245104]
- Faculty of Medical Sciences [93207]
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