Tuning the Surface of Nanoparticles: Impact of Poly(2-ethyl-2-oxazoline) on Protein Adsorption in Serum and Cellular Uptake

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Title:	Tuning the Surface of Nanoparticles: Impact of Poly(2-ethyl-2-oxazoline) on Protein Adsorption in Serum and Cellular Uptake
Author(s):	Koshkina, O.; Westmeier, D.; Lang, T.; Bantz, C.; Hahlbrock, A.; Wurth, C.; Resch-Genger, U.; Braun, U.; Thiermann, R.; Weise, C.; Eravci, M.; Mohr, B.; Schlaad, H.; Stauber, R.H.; Docter, D.; Bertin, A.; Maskos, M.
Publication year:	2016
Source:	Macromolecular Bioscience, vol. 16, iss. 9, (2016), pp. 1287-1300
ISSN:	1616-5187
DOI:	https://doi.org/10.1002/mabi.201600074
Publication type:	Article / Letter to editor
Please use this identifier to cite or link to this item : http://hdl.handle.net/2066/171872
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Subject:	Radboudumc 19: Nanomedicine RIMLS: Radboud Institute for Molecular Life Sciences
Organization:	Tumorimmunology
Journal title:	Macromolecular Bioscience
Volume:	vol. 16
Issue:	iss. 9
Page start:	p. 1287
Page end:	p. 1300
Abstract:	Due to the adsorption of biomolecules, the control of the biodistribution of nanoparticles is still one of the major challenges of nanomedicine. Poly(2-ethyl-2-oxazoline) (PEtOx) for surface modification of nanoparticles is applied and both protein adsorption and cellular uptake of PEtOxylated nanoparticles versus nanoparticles coated with poly(ethylene glycol) (PEG) and non-coated positively and negatively charged nanoparticles are compared. Therefore, fluorescent poly(organosiloxane) nanoparticles of 15 nm radius are synthesized, which are used as a scaffold for surface modification in a grafting onto approach. With multi-angle dynamic light scattering, asymmetrical flow field-flow fractionation, gel electrophoresis, and liquid chromatography-mass spectrometry, it is demonstrated that protein adsorption on PEtOxylated nanoparticles is extremely low, similar as on PEGylated nanoparticles. Moreover, quantitative microscopy reveals that PEtOxylation significantly reduces the non-specific cellular uptake, particularly by macrophage-like cells. Collectively, studies demonstrate that PEtOx is a very effective alternative to PEG for stealth modification of the surface of nanoparticles.