Author(s):
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Koshkina, O.; Westmeier, D.; Lang, T.; Bantz, C.; Hahlbrock, A.; Wurth, C.; Resch-Genger, U.; Braun, U.; Thiermann, R.; Weise, C.; Eravci, M.; Mohr, B.; Schlaad, H.; Stauber, R.H.; Docter, D.; Bertin, A.; Maskos, M.
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Subject:
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Radboudumc 19: Nanomedicine RIMLS: Radboud Institute for Molecular Life Sciences |
Journal title:
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Macromolecular Bioscience
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Abstract:
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Due to the adsorption of biomolecules, the control of the biodistribution of nanoparticles is still one of the major challenges of nanomedicine. Poly(2-ethyl-2-oxazoline) (PEtOx) for surface modification of nanoparticles is applied and both protein adsorption and cellular uptake of PEtOxylated nanoparticles versus nanoparticles coated with poly(ethylene glycol) (PEG) and non-coated positively and negatively charged nanoparticles are compared. Therefore, fluorescent poly(organosiloxane) nanoparticles of 15 nm radius are synthesized, which are used as a scaffold for surface modification in a grafting onto approach. With multi-angle dynamic light scattering, asymmetrical flow field-flow fractionation, gel electrophoresis, and liquid chromatography-mass spectrometry, it is demonstrated that protein adsorption on PEtOxylated nanoparticles is extremely low, similar as on PEGylated nanoparticles. Moreover, quantitative microscopy reveals that PEtOxylation significantly reduces the non-specific cellular uptake, particularly by macrophage-like cells. Collectively, studies demonstrate that PEtOx is a very effective alternative to PEG for stealth modification of the surface of nanoparticles.
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