The Membrane Marker mCLING Reveals the Molecular Composition of Trafficking Organelles
SourceCurrent Protocols in Neuroscience, 74, 2.25, (2016), pp. 1-21
Article / Letter to editor
Display more detailsDisplay less details
Current Protocols in Neuroscience
SubjectRadboudumc 2: Cancer development and immune defence RIMLS: Radboud Institute for Molecular Life Sciences
mCLING is a fixable endocytosis marker that can be combined with immunolabeling techniques to study the molecular composition of trafficking organelles. mCLING can be used both in cultured cells and in tissue if critical sample preparation steps, such as fixation, are correctly performed. This unit describes protocols for the application of mCLING and for the subsequent sample processing. We include immunostaining protocols and embedding procedures for confocal and high-resolution microscopy.
Upload full text
Use your RU credentials (u/z-number and password) to log in with SURFconext to upload a file for processing by the repository team.